Properties of Antisera to Glutamate Dehydrogenase from Nitrogen-fixing Lupin Nodules

Glutamate Dehydrogenase of Lupin Nodules: Purification and Properties

Glutamate dehydrogenase, GDH (L-glutamate:NAD+ oxidoreductase (deaminating) EC 1.4.1.2) was purified from the plant fraction of lupin nodules and the purity of the preparation established by gel electrophoresis and electrofocusing. The purified enzyme existed as 4 charge isozymes with a MW of 270000. The subunit MW, as determined by dodecyl sulphate electrophoresis, was 45000. On the basis of t...

Carbon Partitioning in Nitrogen-fixing Root Nodules

Role of glutamate dehydrogenase in ammonia assimilation in nitrogen-fixing Bacillus macerans.

Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells in which the intracellular NH4+ concentration was 1.4 +/- 0.5 mM, the activit...

Bradyrhizobium ottawaense sp. nov., a symbiotic nitrogen fixing bacterium from root nodules of soybeans in Canada

Sixteen strains of symbiotic bacteria from root nodules of Glycine max grown in Ottawa, Canada, were previously characterized and placed in a novel group within the genus Bradyrhizobium. To verify their taxonomic status, these strains were further characterized using a polyphasic approach. All strains possessed identical 16S rRNA gene sequences that were 99.79 % similar to the closest relative,...

Ferredoxins from Nitrogen-Fixing Bacteria

Two ferredoxins have been purified from N2-fixing Mycobacterium flavum. Ferredoxin I had a minimum molecular weight (by amino acid analysis) of 11 230 and contained 7.4 mol Fe and 7.0 mol S2per mol. The ratio of the absorbance at 400 nm to that at 275 nm was 0.7. Ferredoxin I1 had a minimum molecular weight of 13478 and contained 3.4 mol Fe and 3.2 mol S2per mol. The ratio of the absorbance at ...